Coding

Part:BBa_K5237001:Design

Designed by: Marik Mueller   Group: iGEM24_Heidelberg   (2024-10-01)


Staple subunit: dMbCas12a-Nucleoplasmin NLS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 274
    Illegal PstI site found at 295
    Illegal PstI site found at 607
    Illegal PstI site found at 1762
    Illegal PstI site found at 3049
    Illegal PstI site found at 3324
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 274
    Illegal PstI site found at 295
    Illegal PstI site found at 607
    Illegal PstI site found at 1762
    Illegal PstI site found at 3049
    Illegal PstI site found at 3324
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2225
    Illegal BglII site found at 2351
    Illegal BglII site found at 2858
    Illegal BglII site found at 2904
    Illegal BglII site found at 2951
    Illegal BglII site found at 3227
    Illegal BglII site found at 3311
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 274
    Illegal PstI site found at 295
    Illegal PstI site found at 607
    Illegal PstI site found at 1762
    Illegal PstI site found at 3049
    Illegal PstI site found at 3324
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 274
    Illegal PstI site found at 295
    Illegal PstI site found at 607
    Illegal PstI site found at 1762
    Illegal PstI site found at 3049
    Illegal PstI site found at 3324
    Illegal NgoMIV site found at 355
    Illegal NgoMIV site found at 787
    Illegal NgoMIV site found at 1354
    Illegal NgoMIV site found at 1432
    Illegal NgoMIV site found at 2404
    Illegal NgoMIV site found at 3305
    Illegal NgoMIV site found at 3789
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References